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In addition to providing visual verification of transfer at every step, stain-free imaging enables total protein normalization in each lane. Protein Ladders and Standards Precast Protein Gels Protein Electrophoresis and Blotting Products Supporting Documents and Resources. Visualize total protein on gels and blots using Bio-Rad’s Stain-Free Gels featuring our proprietary Stain-Free Technology. Not intended for human, animal diagnostic or therapeutic uses. To verify protein transfer, stain the membrane with Ponceau S after blotting. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Additional data should be considered for a more accurate adjustment.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. PROTEIN Cell Electrophoresis System Bio-Rad: tank, lid, electrode assembly. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. 2.5 μl per well for general Western transferring. Refer to the Blu13 (BLUelf) Prestained Protein Ladder patterns in various electrophoresis conditions:ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Compare and view all other protein standards and ladders. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Guide for Molecular Weight Estimation (kDa) The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent). The Blu13 Prestained Protein Ladder (BLUelf) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
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The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu13 Prestained Protein Ladder (BLUelf) is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa.